Journal: Advanced healthcare materials

Article Title: Encapsulated mesenchymal stromal cell microbeads promote endogenous regeneration of osteoarthritic cartilage ex vivo

doi: 10.1002/adhm.202002118

Figure Lengend Snippet: (A) Scanned image of the MMPs and TIMPs in the media collected on day 4 as detected and measured using Quantibody® Human Metalloproteinase Antibody Array. (B) Heatmap of z-scores of the quantified MMPs and TIMPs in OA cartilage explants cultured with or without MSC microbeads. Average secreted levels (pg/ml) of (C) MMP1, (D) MMP-2, (E) MMP-3, (F) MMP-8, (G) MMP-9, (H) MMP-10 and (I) MMP-1, (J) TIMP-1, (K) TIMP-2 and (L) TIMP-4 is represented as mean ± standard deviation of 3 OA patient samples. Statistical significance was analyzed by one-way ANOVA with Bonferroni correction on the analytes followed by a post-hoc Tukey’s test and significant comparisons were represented by p-value. *, **, ns indicates p-value <0.05, <0.01 or not significant respectively.

Article Snippet: Multiplexed sandwich-ELISA array Culture media collected on day 4 of culture (in technical duplicates per patient-derived OA per control or co-culture condition) was tested for ECM remodeling-related proteins using Quantibody ® Human Metalloproteinase Antibody Array (QAH-MMP-1-1, Raybiotech) per manufacturer’s instructions.

Techniques: Ab Array, Cell Culture, Standard Deviation

Journal: PLoS ONE

Article Title: Matrix Metalloproteases and Tissue Inhibitors of Metalloproteinases in Medial Plica and Pannus-like Tissue Contribute to Knee Osteoarthritis Progression

doi: 10.1371/journal.pone.0079662

Figure Lengend Snippet: (A) Measurement of human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, and TIMP-4 using a Quantibody ® human MMP ELISA array. The data are the mean ± standard deviation (SD) (N = 15), *P < 0.05. (B) TIMP/MMP ratios in lysates of stage II and stage IV plica (upper panels) and pannus (lower panels) calculated from the Quantibody® human MMP array results. The data are the mean ± SD (N = 15), * P < 0.05.

Article Snippet: To test for 10 MMP-related proteins in lysates of human OA medial plica and pannus-like tissue, an ELISA-based method using Quantibody chips from RayBiotech (Norcross, GA) was used according to the manufacturer's protocol, allowing the simultaneous measurement of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, and TIMP-4 levels.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells

doi: 10.1038/s41598-019-47224-2

Figure Lengend Snippet: Analysis of the ADSC secretome. A profiling of pooled ADSC CM was performed by using an antibody array of 1,000 proteins. Negative control (control medium) was set to 1.0. The relative protein expression in ADSC CM is indicated as x-fold value compared to control medium ( a ). With an array-based multiplex ELISA the concentrations of CCL5, TNF-β, HGF, CXCL12, IFNγ, TNF-α, CXCL1, MMP1, DKK1, FN, THBS1, bFGF, MCP1, ENPP2, TGF- β1, IL-6 and IL-8 were quantified (pg/ml) ( b ).

Article Snippet: For further analyses, an array-based multiplex ELISA (Quantibody ® , RayBiotech Inc./tebu-bio) for chemokine (C-C motif) ligand 5 (CCL5), tumor necrosis factor-beta (TNF-β), hepatocyte growth factor (HGF), stromal cell-derived factor 1 (SDF1)/also known as C-X-C motif chemokine 12 (CXCL12), monocyte chemotactic protein 1 (MCP1)/also known as chemokine (C-C motif) ligand 2 (CCL2), transforming growth factor beta 1 (TGF-β1), C-X-C motif chemokine ligand 1 (CXCL1), basic fibroblast growth factor (bFGF), Dickkopf homolog-1 (DKK1), thrombospondin-1 (THBS1), matrix metalloproteinase-1 (MMP-1) was performed.

Techniques: Ab Array, Negative Control, Expressing, Multiplex Assay, Enzyme-linked Immunosorbent Assay